What is ELISA?(Enzyme-Linked Immunosorbent Assay)

 

What is ELISA? (Enzyme-linked Immunosorbent Assay)

Enzyme-linked Immunosorbent Assay (shortened as ELISA) is used to identify peptides, proteins, antibodies and hormones. Also, called as enzyme immunoassay (EIA), ELISA finds use in the fields of biotechnology and medicine as a diagnostic tool. Mainly, antibodies and color changes are used to identify target substances. Also, ELISAs are useful in measuring antigen and antibody concentration.



ELISA Principle

ELISA works by coupling antibody or antigen to assay enzyme. The assay combines the specificity of antibody and sensitivity of assay enzymes to primarily detect antigens through assay antibody or antibodies through assay antigens. The sensitivity and precision of the assay is enhanced by coating the plate with high-affinity antibodies.

Comments

Post a Comment

Popular posts from this blog

Why D-DIMER test is done ?

Image
A D-dimer test is a simple blood test that can help your healthcare provider determine if you may have a blood clotting condition. If you have a high level of D-dimer in your blood, your provider may have you undergo further blood tests and/or imaging procedures to determine a diagnosis. What is a D-Dimer test? A D-dimer test is a blood tesHaving a high D-dimer level in your blood can be a sign of a blood clotting disorder since the level of D-dimer can rise greatly when there’s significant formation and breakdown of blood clots in your body. That measures D-dimer , which is a protein fragment that your body makes when a blood clot dissolves in your body. D-dimer is normally undetectable or only detectable at a very low level unless your body is forming and breaking down significant blood clots.  A positive or elevated D-dimer test result may indicate that you have a blood clotting condition, but it doesn’t guarantee that you have one. A D-dimer test can't reveal what type of c...
Read more

What is the G6PD test?

Image
  What Is the G6PD Test? The G6PD test is a blood test that measures how much of this enzyme you have in your blood. If you have low amounts, you have a condition called G6PD deficiency.  What Is G6PD Deficiency? G6PD deficiency is a hereditary condition caused by a mutation, or a change, in your G6PD gene. This gene tells your body to make the G6PD enzyme, so a mutation will lower the amount of this useful protein in your body. Click Here - What is Ferritin? Not everyone who has G6PD deficiency has other health problems. The deficiency alone is not enough to cause anemia or problems with your red blood cells. However, individuals who already have this mutation can have new symptoms triggered by outside sources.  These triggers raise the number of reactive oxygen species in your body. When you have an increase in compounds and not enough G6PD to clear them, you can get sick. These triggers include: Eating fava beans Inhaling pollen from a fava pla...
Read more

Best Book For Medical Laboratory Students.

Image
 BEST BOOK FOR MEDICAL     LABORATORY STUDENTS                          Click Here For Buy                                                                  BUY Product Description Medical Laboratory Science: Theory And Practice by J Ochei and A Kolhatkar is a compilation of numerous advancements that have occurred in the field of medical laboratory science in the past few years. The text presents a detailed discussion of theoretical concepts and procedures of each laboratory test. The interpretation and presentation of test results and basic principles are unique and stand-out features of this book. The text is organised into six sections. These are General Approaches to Medical Lab Science, Clinical Chemistry, Haematology, Histopathological...
Read more